|
KMID : 1004820080090020037
|
|
Journal of Biomedical Research
2008 Volume.9 No. 2 p.37 ~ p.46
|
|
|
Protective effect of phytic acid on the formation of aberrant crypt foci induced by azoxymethane in the colon of male ICR mice
|
|
Hue Jin-Joo
Lee Ki-Nam
Miga Myak
Kang Bong-Su
Nam Sang-Yoon
Ahn Byeong-Woo
Yun Young-Won
Lee Beom-Jun
|
|
|
Abstract
|
|
|
|
Phytic acid (PA) is a naturally occurring polyphosphorylated carbohydrate that is present in substantial amounts in almost all plants and mammalian cells. Recently PA has received much attention for its role in anticancer activity. We investigated the preventive effect of PA on the formation of colonic aberrant crypt foci (ACF), a preneoplastic lesion, induced by azoxymethane (AOM). After acclimation for one week, six-week old male ICR mice were fed on the AIN-93G purified diet and PA (0.5% or 2% PA in water) for 8 weeks. The animals were treated with azoxymethane (AOM, 10 mg/kg b.w.) three times (0, 1, and 2 weeks) to induce colonic aberrant crypt foci (ACF). After sacrifice, the total numbers of aberrant crypts (AC) and ACF in colonic mucosa were counted after staining with methylene blue. Blood and serum were analyzed with a blood cell differential counter and an automatic serum analyzer. AOM treatment without PA induced the total numbers of 85.7 ± 14.2 ACF/colon and 132 ± 18.9 AC/colon. The numbers of ACF/colon and AC/colon by PA at the dose of 0.5% were 73.4 ± 12.9 and 115.2 ± 19.9, respectively. PA at the dose of 2% significantly decreased the ACF and AC numbers to 56.5 ± 14.6 and 95.4 ± 17.2, respectively (p<0.01). PA at the doses of 0.5 and 2% decreased the numbers of ACF and AC/colon in a dose-dependent manner. Although some parameters in blood counts and serum chemistry were changed compared with the control, no specific toxicity was found. These findings suggest that phytic acid can be a chemopreventive agent for colon carcinogenesis resulting from inhibition of the development of ACF in ICR mice.
|
|
|
KEYWORD
|
|
|
azoxymethane, aberrant crypt foci, colon cancer, phytic acid
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|